C# 系统可以'；在使用iis访问broswer home.aspx文件时找不到指定文件？

我写了一个asp.net网站，但当我在iis中浏览它时，它提示：

 Exception: System.ComponentModel.Win32Exception: system can't find specify file。

Source error: 


[Win32Exception (0x80004005): The system can't find specify file.]

[SqlException (0x80131904): an instance error associate with network occured when link to SQL Server .Can't find or access to server.Please verify instance name is correct and SQL Server aready configurationa remote link  avaliable . (provider: Named Pipes Provider, error: 40 - can't open SQL Server linker)]
   EcaClient.ECAService.EcaClientService.GetUserModulePermission(Int32 userId, String moduleType) +120
   EcaPortal.CSNavigation.GetCurrentUserModule() in E:\CucEcaPortal\CSNavigation.aspx.cs:113
   EcaPortal.CSNavigation.Page_Load(Object sender, EventArgs e) in E:\CucEcaPortal\CSNavigation.aspx.cs:26
   System.Web.Util.CalliEventHandlerDelegateProxy.Callback(Object sender, EventArgs e) +51
   System.Web.UI.Control.OnLoad(EventArgs e) +92
   System.Web.UI.Control.LoadRecursive() +54
   System.Web.UI.Page.ProcessRequestMain(Boolean includeStagesBeforeAsyncPoint, Boolean includeStagesAfterAsyncPoint) +772

这就是我要做的：

1.Check the connection string,it is aready correct.

2.reconfiguration  the portal in iis.

3.give the portal folder read/write privillege to everyone.  

4.restart the iis

5.Search the error from internet if has any usable suggestion.

注：昨天我的网站是正确的，可以正常运行

w3wp.exe的

w3wp.exe总是显示表单，需要visual 2010来进行调试

当我使用像：
默认应用程序池
这样的用户名检查
w3wp.exe
过程时，这正常吗

为什么iis从其他位置查找路径

我的aspx文件位置是：

E:\appserver\service\CucEcaPortal\CSNavigation.aspx.cs

但它只是从中发现的

E:\CucEcaPortal\CSNavigation.aspx.cs

只需按照以下步骤操作，即可避免此错误：

1.Reset IIS (Start>Run>IIsreset.exe)
2.Clear Cache (Ctrl+Shift+Delete Then Click Clear Now)
3.Clear Temporary File(Start>Run>%Temp% Then Enter,and Delete all Files)
4.Recycle your Website In IIs 
5.Stop/Start SQL Servies( Start>Control Panel>Administrative Panel>Services>Sql Server(Sql Express))

你一定会摆脱这个错误