Bioinformatics Ubuntu 16上的淡紫色

我安装了具有以下功能的Mauve：

apt-get install mauve-aligner

mauveAligner --output=ery.mauve --output-alignment=ery.alignment ery_multiseq.fasta

然后我用以下代码运行它：

apt-get install mauve-aligner

mauveAligner --output=ery.mauve --output-alignment=ery.alignment ery_multiseq.fasta

其中ery_multiseq.fasta是具有我要对齐的不同序列的fasta文件；每个序列都有一个唯一的名称。该文件为13MB，平均序列长度为1.2MB。结果是：

Sequence loaded successfully.
ery_multiseq.fasta 1876490 base pairs.
Sequence loaded successfully.
ery_multiseq.fasta 1876490 base pairs.
Sequence loaded successfully.
ery_multiseq.fasta 1752910 base pairs.
Sequence loaded successfully.
ery_multiseq.fasta 1752910 base pairs.
Sequence loaded successfully.
ery_multiseq.fasta 1787941 base pairs.
Sequence loaded successfully.
ery_multiseq.fasta 1787941 base pairs.
Sequence loaded successfully.
ery_multiseq.fasta 346770 base pairs.
Sequence loaded successfully.
ery_multiseq.fasta 398993 base pairs.
Sequence loaded successfully.
ery_multiseq.fasta 454198 base pairs.
Sequence loaded successfully.
ery_multiseq.fasta 731061 base pairs.
Using 15-mers for initial seeds
Creating sorted mer list
Create time was: 1 seconds.
Creating sorted mer list
Create time was: 1 seconds.
Creating sorted mer list
Create time was: 0 seconds.
Creating sorted mer list
Create time was: 1 seconds.
Creating sorted mer list
Create time was: 1 seconds.
Creating sorted mer list
Create time was: 1 seconds.
Creating sorted mer list
Create time was: 0 seconds.
Creating sorted mer list
Create time was: 1 seconds.
Creating sorted mer list
Create time was: 0 seconds.
Creating sorted mer list
Create time was: 0 seconds.
0%..1%..2%..3%..4%..5%..6%..7%..8%..9%..10%..
11%..12%..13%..14%..15%..16%..17%..18%..19%..20%..
21%..22%..23%..24%..25%..26%..27%..28%..29%..30%..
31%..32%..33%..34%..35%..36%..37%..38%..39%..40%..
41%..42%..43%..44%..45%..46%..47%..48%..49%..50%..
51%..52%..53%..54%..55%..56%..57%..58%..59%..60%..
61%..62%..63%..64%..65%..66%..67%..68%..69%..70%..
71%..72%..73%..74%..75%..76%..77%..78%..79%..80%..
81%..82%..83%..84%..85%..86%..87%..88%..89%..90%..
91%..92%..93%..94%..95%..96%..97%..98%..99%..Starting with 43306 MUMs
Eliminating overlaps yields 43767 MUMs
Multiplicity filter gives 0 MUMs

ery.mauve文件包含：

FormatVersion   4
SequenceCount   10
Sequence0File   ery_multiseq.fasta
Sequence0Length 1876490
Sequence1File   ery_multiseq.fasta
Sequence1Length 1876490
Sequence2File   ery_multiseq.fasta
Sequence2Length 1752910
Sequence3File   ery_multiseq.fasta
Sequence3Length 1752910
Sequence4File   ery_multiseq.fasta
Sequence4Length 1787941
Sequence5File   ery_multiseq.fasta
Sequence5Length 1787941
Sequence6File   ery_multiseq.fasta
Sequence6Length 346770
Sequence7File   ery_multiseq.fasta
Sequence7Length 398993
Sequence8File   ery_multiseq.fasta
Sequence8Length 454198
Sequence9File   ery_multiseq.fasta
Sequence9Length 731061
IntervalCount   0

而ery.alignment是完全空的

我做错了什么

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非常感谢

PS：启动GUI版本时，我遇到了一个错误：“序列可能没有任何同源区域”，这很愚蠢，因为序列来自同一物种的变体。是否可能某些序列与其他序列相反？如果是这样，这是否会导致GUI版本出现问题？（我不知道mauve应该做什么。可能它会自动处理这个问题，我的评论与此无关。）注意，现在有一个beta生物信息学stackexchange网站，在那里这个问题可能会得到答案：PS：启动GUI版本我得到错误：“序列可能没有任何同源区域”既然这些序列来自同一物种的变体，那又有什么愚蠢的呢？有没有可能某些序列相对于其他序列是反向互补的呢？如果是这样，这是否会导致GUI版本出现问题？（我不知道mauve应该做什么。也许它会自动处理这个问题，而我的评论与此无关。）请注意，现在有一个beta生物信息学stackexchange网站，在那里这个问题可能会得到答案：