如何解决这些错误：x的插槽需要使用唯一的名称填充，或者下标包含越界索引，使用predORF？

我对这个函数predORF有一些问题

  sqa <- c("CAGGGCACCTGGCCTTGGGATGCGCCTCCTGCCCGCTGAGCCCAGGGGCCGCTATGGCCCTTCTGGCCATGCTGGCGCTGCAGACAGCTCTCTACCTAGTAGGCTTCTTCTACCCGCCGGGAGGCATATGGCGCTGGATCACCCGGGAC") 
  print(sqa)   

我犯了这个错误

  Error in predORF(dnastring, n = 1, type = "grl", mode = "orf", strand = "sense",  : 
  Sequence name slot of x need be populated with unique names.
    

  Error in predORF(dna n = 1, type = "grl", mode = "orf", strand = "sense",  : 
  Sequence name slot of x need be populated with unique names.
    

当我定义它的长度时，我得到：

    predORF(dna[:149], n = 1, type = "grl", 
    mode = "orf", strand = "sense", 
    longest_disjoint=FALSE, 
    startcodon = c("ATG"), 
    stopcodon = c("TAA"))

  Error in predORF(dnastring[1:149], n = 1, type = "grl", mode = "orf",  : 
  Sequence name slot of x need be populated with unique names.

    predORF(dna[:149], n = 1, type = "grl", 
    mode = "orf", strand = "sense", 
    longest_disjoint=FALSE, 
    startcodon = c("ATG"), 
    stopcodon = c("TAA"))

  Error: subscript contains out-of-bounds indices

当我使用dnastingset格式时：

    predORF(dna, n = 1, type = "grl", 
    mode = "orf", strand = "sense", 
    longest_disjoint=FALSE, 
    startcodon = c("ATG"), 
    stopcodon = c("TAA"))

我犯了这个错误

  Error in predORF(dnastring, n = 1, type = "grl", mode = "orf", strand = "sense",  : 
  Sequence name slot of x need be populated with unique names.
    

  Error in predORF(dna n = 1, type = "grl", mode = "orf", strand = "sense",  : 
  Sequence name slot of x need be populated with unique names.
    

当我定义它的长度时，我得到：

    predORF(dna[:149], n = 1, type = "grl", 
    mode = "orf", strand = "sense", 
    longest_disjoint=FALSE, 
    startcodon = c("ATG"), 
    stopcodon = c("TAA"))

  Error in predORF(dnastring[1:149], n = 1, type = "grl", mode = "orf",  : 
  Sequence name slot of x need be populated with unique names.

    predORF(dna[:149], n = 1, type = "grl", 
    mode = "orf", strand = "sense", 
    longest_disjoint=FALSE, 
    startcodon = c("ATG"), 
    stopcodon = c("TAA"))

  Error: subscript contains out-of-bounds indices

我如何解决这个问题

---

提前谢谢

问题是predORF需要命名序列作为输入。见下文：

library(Biostrings)
library(systemPipeR)

sqa <- c("ATGTAA")
sqb <- c("ATGGCCTAA")
dna <- DNAStringSet(c(seq_a = sqa, seq_b = sqb), use.names = T) 
predORF(dna, n = 1, type = "grl", 
        mode = "orf", strand = "sense", 
        longest_disjoint=FALSE, 
        startcodon = c("ATG"), 
        stopcodon = c("TAA"))

库（生物串）
图书馆（systemPipeR）
sqa